Supplemental information S5 110830.cdr
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چکیده
Design of two RTQPCRs for XMRV-like murine leukemia viruses. © program The variation in MLV gag genes was studied by blasting the nr portion of GenBank with gag of XMRV VP62. BLASTN retrieved 109 MLV gag sequences. The result was treated with the variation analysis program ConSort (Blomberg et al, unpublished) (fig S1 A and B). The selected portion of gag corresponded approximately to the major homology region of Gag. Figure S5 A. ConSort representation of variation in the entirety of MLV-like gag sequences. The selected gag primers and probe are shown above the variation histogram. Red bars indicate sequence portions containing at least one invariant nucleotide position. Figure S5. B. A close-up view of the ConSort representation of the gag portion selected for primer and probe construction. ConSort of entire MLV-like gag sequences. The mauve rectangles at the top indicate regions suggested as primer or probe targets by ConSort.
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